In January 2008, Wayne recorded two videos addressing her friends on Gaia Online and uploaded them to YouTube under the alias boxxybabee. They were reposted to the site i-am-bored.com later in the year, and then eventually appeared on 4chan. Wayne responded to the popularity of her initial videos in a third video that was uploaded to her new YouTube channel, boxxybabee, in January 2009. The videos depict Wayne in heavy eyeliner rambling excitedly about various things.

In March 2010, Urlesque named Boxxy number 104 on its list of "The 100 Most Iconic Internet Videos". She was left out of the top 100 list because her videos were relatively new at the time. In late 2010, Wayne began selling Boxxy items on eBay. On November 25, 2010, she uploaded an edited clip from the film Star Wars Episode IV: A New Hope under the alias ANewHopeee. The original clip contained Princess Leia's holographic message to Obi-Wan Kenobi. Wayne replaced Princess Leia's speech with her own request for her fans' help in regaining access to her old account, boxxybabee.

On June 17, 2011, Wayne made a third YouTube account (Bodaciousboxxy) and continued uploading videos of herself. In this video she claims that the Boxxy in the video of January 2011 is not the real Boxxy, but a girl who looks a lot like her, who was supposedly hacked by a character named "Svetlana".

Between December 2013 and December 2014, Wayne was a host for the Animalist streaming series for Discovery Digital Networks.

In 2017, Wayne voiced the character Marsha for Disney XD's Billy Dilley's Super-Duper Subterranean Summer.