Stefanie Martini made her debut in an episode of the ITV series Endeavour. At the time, she was still in her third year at RADA. In 2016, she starred as Mary Thorne in the ITV series Doctor Thorne and as Princess Langwidere in the NBC series Emerald City.
Later that year, she portrayed Jane Tennison in the popular ITV series Prime Suspect 1973. In the same year, she starred in the film Crooked House as Sophia de Haviland. Martini played Phyllis Lambert in the 2018 film Hurricane aka Mission of Honor, alongside Iwan Rheon and Milo Gibson.
Martini is most known for her role as Eadith in the acclaimed history television series The Last Kingdom (2020-21), in which she co-starred with renowned actors Alexander Dreymon, Emily Cox, and Eliza Butterworth.